Abstract A rapid, sensitive, selective and reliable strip assay based on DNA-functionalized gold nanoparticles for Hg2+ detection has been developed, with a detection limit 5 nmol/L. The measurement principle was based on thymine-Hg2+-thymine (T-Hg2+-T) coordination chemistry and streptavidin-biotin interaction. The major advantages of this assay are that results can be read visually without any instrument in less than 10 min and that it does not require any sample pretreatment.
Received: 14 July 2011
Fund:Financial supports from Science and Technology Department of Zhejiang Province of China (No. 2010C33004), Department of Education of Zhejiang Province (No. Y201010064) are gratefully acknowledged. This work was also sponsored by K.C. Wong Magna Fund in Ningbo University.