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| Glycine-conjugated porphyrin fluorescent probe with iRGD for live cell imaging |
| Qian Zhanga,b, Xue Donga,b, Kun-Peng Wanga,b, Ting-Ting Zhua,b, Feng-Nan Suna,b, Shu-Xian Menga,b, Ya-Qing Fenga,b |
a School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
b Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin 300072, China |
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Abstract A porphyrin modified by glycine has been synthesized and developed as a near-infrared(NIR) fluorescence probe to detect tumor.Porphyrins'longwavelength emission at ~650 nm can efficiently avoid the spectral crosstalk with Spontaneous fluorescence in the visible light region.A disulfide-based cyclic RGD peptide named iRGD c(CRGDKGPDC),a tumor homing peptide,harbors a cryptic C-end Rule (CendR)motif that is responsible for neuropilin-1(NRP-1)binding and for triggering extravasation and tumor penetration of the peptide to improve the imaging sensitivity and therapeutic efficacy.We used N-hydroxy succinimide as an activator to introduce the glycine methyl ester to detect tumor.We got a porphyrin modified by glycine.The affinity between probe and tumor cell entered GLC-82 cells(human glandular lung cancer cell line)can be observed by Confocal Microscope.The toxicity of probe has been identified by MTT Assay.The summary has been gotten that the porphyrins were nontoxic to GLC-82 cells and glycine modified porphyrin has a good affinity with GLC-82 cells under the iRGD function by our experiment.
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Received: 03 January 2017
Published: 04 March 2017
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| Fund:This work is supported by National Natural Science Foundation of China(No.21076147).We are grateful to Prof.H.Huang and Prof. L.Yang for providing GLC-82 cells and some support. |
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Corresponding Authors:
Shu-Xian Meng
E-mail: msxmail@tju.edu.cn
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