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| Sequence identification, structure prediction and validation of tannase from Aspergillusniger N5-5 |
| Shuai Zhanga,b, Feng-Chao Cuic, Yong Caoa, Yun-Qi Lic |
a College of Food Science, South China Agricultural University, Guangzhou 510642, China;
b College of Chemistry and Chemical Engineering, Zhaoqing University, Zhaoqing 526061, China;
c Key Laboratory of Synthetic Rubber, Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022, China |
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Abstract Tannases produced by filamentous fungi are in a family of important hydrolases of gallotannins and have broad industry applications. But until now, the 3-D structures of fungi tannases have not been reported. The protein sequence deduced from the cDNA sequence obtained using RT-PCR amplification was identified as tannase through sequence alignment and phylogenetic analysis. Structure models based on the tannase sequence were collected using I-TASSER, and the model with the best match to the surface charge density-pH titration profile was selected as the final structure for tannase from Aspergillusniger N5-5. This work provides an effective method for protein structure research. The structure constructed in this work should be very important to understand the enzyme bioactivities and further developments of fungi tannases.
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Received: 15 December 2015
Published: 23 April 2016
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| Fund:The authors are grateful to the National Natural Science Foundation of China (No. 21374117) and the 100 Talents Program of Chinese Academy of Sciences for financial support. |
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Corresponding Authors:
Yong Cao
E-mail: caoyong2181@scau.edu.cn
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