|
|
|
| Simultaneous kinetic spectrophotometric determination of cephalexin and trimethoprim in pharmaceutical preparation and human urine with the aid of chemometrics |
| Yong Nian Nia,b, Wei Qiang Xiaob |
a The Stake Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
b Department of Chemistry, Nanchang University, Nanchang 330047, China |
|
|
|
|
Abstract A procedure for the simultaneous kinetic spectrophotometric determination of cephalexin and trimethoprim was described.It was based on the different reaction rate of oxidation of these compounds with yellow ammonium cerous (Ⅳ) sulfate in acidic medium and colorless cerous (Ⅲ) sulfate was produced.The overlapped kinetic data was quantitatively resolved by the use of chemometric methods, partial least squares (PLS), principal component regression (PCR) and radial basis function-artificial neural network (RBF-ANN).The proposed method was also applied to the simultaneous determination of cephalexin and trimethoprim in pharmaceutical preparation and human urine with satisfied results, which compared well with those obtained by HPLC.
|
|
Received: 21 March 2008
|
| Fund:The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (No.20562009);the Natural Science Foundation of Jiangxi Province (No.0620041);the State Key Laboratory of the Chemo/Biosensing and Chemometrics of Hunan University (No.2005-22);and the program for Changjiang Scholars and Innovative Research Team in Universities (No.IRT0540) |
|
Corresponding Authors:
Yong Nian Ni, E-mail address:ynni@ncu.edu.cn
E-mail: ynni@ncu.edu.cn
|
|
|
|
|
|
|
