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| Recognizing parallel-stranded G-quadruplex by cyanine dye dimer based on dual-site binding mode |
| Li-Jia Yua,b, Wei Gaia,b, Qian-Fan Yanga, Jun-Feng Xianga, Hong-Xia Suna, Qian Lia, Li-Xia Wanga, Ai-Jiao Guana, Ya-Lin Tanga |
a Beijing National Laboratory for Molecular Science, Center for Molecular Science, State Key Laboratory for Structural Chemistry for Unstable and State Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
b University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract G-quadruplexes (G4s) play important roles in biological systems, such as telomere maintenance, replication, and transcription. Based on the DNA sequence, loop geometry, and the local environments, G4s can be classified into different conformations. It is important to detect different types of G4s to monitor the diseases related with G4s. Most ligands bind to G4s based on end-stacking modes, while rare ligands bind to G4s through groove binding modes. We have found that a cyanine dye DMSB interacts with parallel G4 by end-stacking and groove simultaneous binding mode. In this article, we found that DMSB could simply discriminate parallel G4s from other DNA motifs by using UV-vis spectrum. These results give some clues to develop high specificity G4 probes.
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Received: 23 October 2014
Published: 25 February 2015
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| Fund: This present study was supported by Major National Basic Research Projects (973, No. 2013CB733701), National Natural Science Foundation of China (Nos. 81072576, 91027033, 21302188, 21205121, 21305145 and 31200576), and Chinese Academy of Sciences (No. KJCX2-EW-N06-01). |
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Corresponding Authors:
Qian-Fan Yang, Ya-Lin Tang
E-mail: yangqf@iccas.ac.cn;tangyl@iccas.ac.cn
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