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| Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system |
| Wen Juan Donga, Jin Ping Songa, Chuan Donga, Martin M. F. Choib |
a Research Center of Environmental Science and Engineering, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
b Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China |
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Abstract The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0×10-7-1.0×10-3 mol L-1 and a detection limit 1.0×10-7 mol L-1 of catechol were obtained, o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching.
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Received: 17 August 2009
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| Fund:This work was supported by the National Natural Science Foundation of China (No. 20875059). |
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Corresponding Authors:
Chuan Dong,E-mail address:dc@sxu.edu.cn
E-mail: dc@sxu.edu.cn
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