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| Highly selective electrochemical method for the detection of serotonin at carbon fiber microelectrode modified with gold nanoflowers and overoxidized polypyrrole |
| Jiajia Songa, Lifen Wanga, Hetong Qib, Honglan Qia, Chengxiao Zhanga |
a Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China;
b Department of Applied Chemistry, School of Science, Xi'an Jiaotong University, Xi'an 710049, China |
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| Guide A gold nanoflowers and overoxidized polypyrrole modified carbon fiber microelectrode (OPPy/Au NFs/CFME) was fabricated using electroless deposition and electrochemical method for highly selective and sensitive detection of 5-HT. |
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Abstract A highly selective and sensitive electrochemical method was developed for the detection of serotonin (5-hydroxytryptamine, 5-HT) at gold nanoflowers (Au NFs) and overoxidized polypyrrole (OPPy) modified carbon fiber microelectrode (CFME). Carbon fiber was firstly modified with gold nanoflowers using electroless deposition method, and then modified with overoxidized polypyrrole using electrochemical polymerization and overoxidization to obtain OPPy/Au NFs/CFME. The obtained OPPy/Au NFs/CFME was characterized by field emission scanning electron microscopy and electrochemical techniques. It was found that the OPPy/Au NFs/CFME showed good sensitivity for the detection of 5-HT in the range of 10 nmol/L-7.0 μmol/L with detection limit of 2.3 nmol/L, and negligible interferences from ascorbic acid, 5-hydroxyindole acetic acid and uric acid. The OPPy/Au NFs/CFME was successfully applied to the detection of 5-HT in human serum samples with good recovery. The work demonstrates that the electrochemical method, incorporating signal amplification of Au NFs with higher cation selection of OPPy, provides a promising tool for the detection of 5-HT in biological systems
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Received: 18 April 2019
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| Fund:We thank the National Natural Science Foundation of China (Nos. 21775097 and 21804106), the China Postdoctoral Science Foundation (No. 2017M620444) and the Fundamental Research Funds for the Central Universities (Nos. XJJ2018247 and GK201801006). |
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